Effect of casein phosphopeptide-amorphous
calciumphosphate paste on hardness of
enamel exposed to chlorinated water: an
in vitro study
Authors :
- Tharinee Phanomroengsak D.D.S.
- Sommai Chobisara D.D.S., Grad. Dip. in Pediatric Dentistry, Thai Board of Pediatric Dentistry
- Suchit Poolthong D.D.S., Grad. Dip. in Operative Dentistry, M.Sc., Ph.D.
Abstract :
Objective The purpose of this study was to assess the effect of casein phosphopeptide-amorphous
calciumphosphate paste on intact enamel exposed to chlorinated water.
Materials and methods Twelve human premolars extracted for orthodontic reason were prepared for
hardness measurement using nanoindentation system. The specimens were randomly divided into two
groups. The experimental group was applied with casein phosphopeptide-amorphous calciumphosphate
paste on intact enamel surface for three minutes and immersed in artificial saliva for 30 minutes while
the control group was left unapplied. Hardness in giga Pascal was measured before and after being
exposed to chlorinated water (pH 5.3) for 2 hours and artificial saliva for 8 hours. The data were
analyzed by Wilcoxon signed rank test (p = 0.05).
Results The results showed that the hardness of the intact enamel before and after exposure to
chlorinated water (pH 5.3) for 2 hours and artificial saliva for 8 hours was insignificantly changed in
both of the control group (4.34 ± 0.92 and 4.38 ± 0.72 GPa) and experimental group (4.91 ± 0.32
and 5.05 ± 1.29 GPa).
Conclusion We concluded that casein phosphopeptide-amorphous calciumphosphate paste did not
have any effect on enamel hardness exposed to chlorinated water (pH 5.3) for 2 hours and artificial
saliva for 8 hours in vitro.
(CU Dent J. 2009;32:203-12)
Keywords :
casein phosphopeptide-amorphous calciumphosphate; chlorinated water; enamel;
hardness; nanoindentation system
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