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Article

Effects of dental radiation on the expression of apoptotic-related genes in primary human bone cells

Authors :
  • Sakarat Pramojanee D.D.S.
  • Prasit Pavasant D.D.S., Ph.D.
  • Soontra Panmekiate D.D.S., M.D.Sc., Odont.

Abstract :

Objective To investigate the effects of dental radiation on the expression of apoptotic-related genes
in primary human bone cells.

Materials and methods Human bone cells were grown in 15% FBS-DMEM and irradiated with 0, 1,
2 doses of a periapical radiograph. The cytotoxicity of irradiation was investigated by MTT assay after
24 hours. The levels of apoptotic-related gene expressions, Bcl-2, Bax, Bad, Bcl-xL, caspase-3 were
analyzed by reverse transcription polymerase chain reaction, four hours after irradiation.

Results No cytotoxicity was observed at the time of 24 hours after dental irradiation. The results
indicated that Bax and caspase-3 significantly decreased after irradiation as compared to the control
group, whereas, the expression of Bcl-2, Bad and Bcl-xL did not change significantly after dental
irradiation.

Conclusion Dental radiation affects apoptotic-related gene expressions. Therefore, prescribing dental
radiograph should be highly selective and the principle of ALARA (As Low As Reasonably Achievable)
should be strictly followed.

(CU Dent J. 2008;31:415-25)



Keywords :
apoptosis; Bcl-2 family; caspase-3; dental radiation; human bone cells
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