• Chureerat Kanchanakaew D.D.S.
• Somsinee Pimkhaokham D.D.S., Ph.D.
• Jeerus Sucharitakul D.D.S., Ph.D. (Biochemistry)

Objective The purpose of this in vitro study was to compare the leakage of roots filled with Epiphany^®
and Resilon^® after final irrigation with 2% chlorhexidine.

Materials and methods Seventy single-rooted teeth were prepared using crown down technique
and divided to three experimental groups of twenty samples each and positive and negative control
groups of five samples each. In each experimental group, root canal was irrigated with 2.5% sodium
hypochlorite and 17% EDTA then final irrigated with sterile water (group 1), 2% chlorhexidine
(group 2) or 2.5% sodium hypochlorite followed by 2% chlorhexidine (group 3). The experimental
groups were filled with laterally compacted Resilon^® cones and Epiphany^® sealer. The leakage of
glucose was evaluated by measuring its concentration once a week for a total period of 28 days
using a glucose penetration model. Data of glucose concentration that leaked through the filled
root canals were not normally distributed. Therefore, they were statistically analyzed by the nonparametric
test (Friedmann test and Kruskal-Wallis test). The level of significance was set at p = .05.

Results The increasing glucose concentration values among each time intervals in the same
experimental group were statistically significant different. However, no statistically significant
differences were found among the three experimental groups at each time interval.

Conclusion This study showed that 2% chlorhexidine when used after 17% EDTA and 2.5% sodium
hypochlorite did not adversely affect the leakage of root canal system filled with Epiphany^®
and Resilon^®.

(CU Dent J. 2008;31:135-44)