• Vichitra Vipismakul D.D.S. Dr.med.dent.
• Pasutha Thunyakitpisal D.D.S., Ph.D.

Objective To investigate the effect of aloe vera gel extract and exudate on the proliferation of
osteoblasts isolated from the rat bone marrow stromal cells and the osteoblastic cell line MC3T3-E1.

Materials and methods Cells were treated with the aloe vera gel extract and exudate at different
concentrations in the serum-free media for 24 hours. After that the amount of cells were measured with
the MTT assay and analyzed with One-Way Analysis of Variance.

Results The aloe vera gel extract, at concentrations of 10, 20 and 50 μg/ml, significantly enhanced
the proliferation of osteoblasts isolated from the rat bone marrow (p < .05). The aloe vera gel extract,
at concentrations of 1, 5, 10, 20 and 50 μg/ml, significantly induced the proliferation of osteoblastic
cell line MC3T3-E1 (p < .05). In contrast, the aloe vera exudate, at concentrations of 20, 40 and 60
μg/ml, significantly decreased the proliferation of osteoblasts isolated from the rat bone marrow and
the osteoblastic cell line MC3T3-E1 (p < .05).

Conclusion The aloe vera gel extract, 10-50 and 1-50 μg/ml, significantly induced the proliferation
of the osteoblasts isolated from the rat bone marrow and the osteoblastic cell line MC3T3-E1, respectively.
In contrast, the aloe vera gel exudates, 20-60 μg/ml, significantly decreased the cell number of
both osteoblasts isolated from the rat bone marrow and the osteoblastic cell line MC3T3-E1.

(CU Dent J. 2005:28:127-36)