Fluoride promotes human pulp cell proliferation through p38 kinase pathway
Authors :
- Kritchai Bespinyowong
- Prasit Pavasant
Abstract :
Objective To study the effects of fluoride on cell proliferation in cultured human pulp cells and investigate the mechanism of fluoride on cell proliferation.
Materials and methods Effect of fluoride on cell proliferation was analysed by methylene blue and MTT assay. For determining the intracellular pathway, cells were treated with fluoride in the presence of MAPK inhibitors. The results were statistically analysed by using One-way Analysis of Variance.
Results The cytotoxic effect of fluoride on human pulp cells was observed at ≥ 25 ppm (p < 0.05). In contrast, 1 and 10 ppm of fluoride significantly stimulated dental pulp cell proliferation (p < 0.05). The proliferative effect of fluoride may occur through p38 kinase since the inhibitor of p38 kinase inhibited fluoride-induced dental pulp cell proliferation.
Conclusion Fluoride concentration higher than 25 ppm has cytotoxic effect on human pulp cells, whereas fluoride at 1 and 10 ppm promote human pulp cell proliferation via p38 kinase.
(CU Dent J. 2006;29:63-74)
Keywords :
fluoride; p38 kinase; pulp cells
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