Effect of simvastatin on the proliferation and enzyme activity of MMP-2 and -9 in cancer cell lines HSC-4 and CCL-25
Authors :
- Risa Chaisuparat
- Phakawan Musikapong
- Pasutha Thunyakitpisal
Abstract :
Objective To study the effect of simvastatin on the proliferation and enzyme activity of MMP-2 and -9 in HSC-4 and CCL-25 cancer cell lines
Materials and methods Cells were treated with simvastatin in the serum-free media for 24 hours. The number of cells were measured by MTT technique. Enzyme activities in the conditioned media were measured by gelatin zymography. The data were analyzed by One way ANOVA (α<0.05).
Results Simvastatin, at 15 and 20 µM, significantly decreased the proliferation of HSC-4 and CCL-25 compared with the control group. Simvastatin, at 10 µM, significantly decreased the MMP-9 activity in CCL-25. Simvastatin did not significantly effect the MMP-2 and MMP-9 activities in HSC-4.
Conclusion In HSC-4 and CCL-25, the toxicity concentration of simvastatin is 15 and 20 µM. Simvastatin, at 10 µM, significantly suppressed MMP-9 activity in CCL-25.
(CU Dent J. 2004;27:59-67)
Keywords :
gelatin zymography; MMP-9; simvastatim; toxicity test
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