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Pulpal blood flow in streptozotocin-induced diabetic rats measured by laser Doppler flowmetry
Authors :
- Supathra Amatyakul
- Daroonwan Chakraphan
- Siriporn Chotipaibulpan
- Suthiluk Patumraj
Abstract :
Objective The aim of this study is to investigate the pulpal blood flow in streptozotocin-induced diabetic rats by using laser Doppler flowmeter
Materials and methods The animal model of streptozotocin (STZ)-induced diabetic rats (i.v. injection of STZ 55 mg/kg BW) was used. Thirty-two male Spraguep-Dawley rats weighing 200-250 g were divided equally into 2 groups; non-diabetes (CON) and diabetes (STZ). At 12 weeks (wks) and 24 wks after the STZ injection, the laser Doppler flowmeter (Model ALF 21, USA.) was used to measured pulpal blood flow of the right lower incisor while the animals were anesthetized with an intraperitoneal injection of sodium pentobarbital (50 mg/kg BW).
Results The present study demonstrated that STZ-rats developed hyperglycemia (blood glucose, mg/dl: CON-rats = 85.13 + 8.69, STZ-ratz = 402.88 + 24.81 at 12 wks; CON-rats = 98.00 + 5.11, STZ-rats = 327.75 + 16.08 at 24 wks), higher glycosylated hemoglobin levels (glycosylated hemoglobin,%: CON-rats = 3.68 + 0.05, STZ-rats = 10.86 + 0.24 at 12 wks; CON-rats = 3.41 + 0.09, STZ-rats = 11.20 + 0.38 at 24 wks), higher mean arterial blood pressure (mean arterial blood pressure, mmHg: CON-rats = 93.75 + 6.48, STZ-rats = 114.50 + 2.80 at 12 wks; CON-rats = 87.29 + 2.44, STZ-rats = 115.00 + 8.09 at 24 wks), and loss of body weight (body weight,g: CON-rats = 424.88 + 5.08, STZ-rats = 183.38 + 8.87 at 12 wks; CON-rats = 487.88 + 13.52, STZ-rats = 333.38 + 28.15 at 24 wks) Plasma level of vitamin C in STZ-rats was significantly lower than CON-rats (plasma vitamin C,g/l: CON-rats = 1.30 + 0.15, STZ-rats = 0.62 + 0.02 at 12 wks; CON-rats = 1.24 + 0.10, STZ-rats = 0.65 + 0.08 at 24 wks). The decrease of pulpal blood flow in intact right lower incisor was observed in STZ-rats (pulpal blood flow,ml/min/100g tissue: CON-rats = 30.40 + 1.95, STZ-rats = 16.73 + 2.77 at 12 wks; CON-rats = 29.54 + 3.08, STZ-rats = 16.09 + 1.58 at 24 wks).
Conclusion By using laser Doppler flowmeter, the present study shows the decrease of pulpal blood flow in streptozotocin-induced diabetic rats.
(CU Dent J 2003;26:221-7)
Keywords :
laser Doppler flowmetry; pulpal blood flow; rats; streptozotocin-induced diabetes
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