Inhibitory effect of EGCG of EGCG on collagen induced MMP-2 activation in oral squamous carcinoma cell lines
Authors :
- Neeracha Sanchavanakit
- Amornrat Suwanchai
- Orachatr Boonyakiati
- Artiphan Pimkhaokham
- Prasit Pavasant
Abstract :
Objective The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG), the major polyphenol compound of the tea extract on the inhibition of matrix metalloproteinase (MMP)-2 activation in oral squamous carcinoma cell lines.
Materials and methods The study was performed in the cultures of human squamous carcinoma (HSC) -3 and 7 and human foreskin fibroblast (HFF). EGCG at 0, 1 and 10 µM was added to the 200 µg/ml collagen induced MMP-2 activation in these cell lines of 48 hours. Conditioned media were collected and MMP-2 activation was analyzed by gelatin zymography.
Result Ten µM EGCG reduced the activation of MMP-2 in HSC-3 and HFF, whereas inhibited that of MMP-2 in HSC-7.
Conclusion The result suggested another role of EGCG in the reduction of MMP-2 activation, which was implicated in cancer metastatic process, apart from an antioxidant effect on carcinogenesis that has been reported in various types of cancer.
(CU Dent J 2003;26:1-14)
Keywords :
EGCG; HSC; MMP-2 activation
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