An Inverse Relationship between A
Disintegrin and Metalloproteinase-9
and Proliferating Cell Nuclear Antigen
Expression in Oral Lichen Planus
Authors :
- Darussakorn Maneerat D.D.S.
- Pareena Chotjumlong Ph.D.
- Thanapat Sastraruji Ph.D.
- Surawut Pongsiriwet D.D.S., M.D.Sc.
- Anak Iamaroon D.D.S., M.S., Ph.D.
- Suttichai Krisanaprakornkit D.D.S., M.S.D., Ph.D.
Abstract :
Background A Disintegrin and Metalloproteinase-9 (ADAM-9) is a type I transmembrane protein
associated with epithelial differentiation. ADAM-9 is involved in wound healing process by controlling
keratinocyte migration and tumorigenesis. Proliferating cell nuclear antigen (PCNA) is a useful marker
for cell proliferation. Several studies reported increased PCNA expression in oral lichen planus (OLP),
but ADAM-9 expression in OLP has not yet been studied.
Objectives To compare the expression of ADAM-9 and PCNA in OLP with that in normal oral
mucosa and to find correlations between ADAM-9 or PCNA expression and OLP clinical severity.
Materials and Methods The expression of ADAM-9 and PCNA was studied in 25 OLP and thirteen
normal specimens by immunohistochemistry. The clinical severity of patients with OLP was assessed
by a clinical criteria score. Non-parametric statistical tests were used to determine differences in
ADAM-9 and PCNA expression between OLP and normal specimens and correlations between
ADAM-9 or PCNA expression and OLP severity.
Results ADAM-9 expression was significantly decreased in OLP epithelium compared with normal oral
epithelium (p<0.001) and inversely correlated with the clinical criteria score (r=-0.476; p<0.01), whereas
PCNA expression was significantly increased in OLP epithelium (p<0.001) and positively correlated with
the clinical criteria score (r=0.817; p<0.001). Moreover, ADAM-9 expression was inversely correlated with
PCNA expression (r=-0.444; p<0.05).
Conclusions Decreased ADAM-9 expression in OLP epithelium suggests reduction of epithelial differentiation,
whereas enhanced PCNA expression in OLP epithelium suggests induced epithelial proliferation.
The differential degrees of ADAM-9 and PCNA expression may be used as prognostic markers for OLP
severity.
(CU Dent J. 2015;38(Suppl):1-12)
Keywords :
A disintegrin and metalloproteinase domain 9; Biological Markers; Immunohistochemistry;
Oral lichen planus; Proliferating cell nuclear antigen
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