Effect of aloe vera gel and exudate extracts on the proliferation of primary cultured gingival fibroblasts and keratinocyte, In vitro
Authors :
- Pasutha Thunyakitpisal
- Damrong Damrongsri
- Narumol Charearnwetchatom
- Supeepan Boonyaratanasoontorn
- Sureerut Udomkittanasarn
Abstract :
Objective To study the effect of aloe vera extracts on the proliferation of gingival fibroblasts and ketatinocytes CCL-25
Material and methods Cells were treated with the different protein concentration of aloe vera gel and exudate extracts for 24 hours. After that the number of cells were measured by the methylene blue staining assay. The results were statistically analyzed by using One-way Analysis of Variance.
Results The aloe vera gel extract at protein concentration of 1-50 µg/ml induced the gingival fibroblast proliferation significantly, but not keratinocyte. The exudate extract at protein concentration of 20-400 µg/ml significantly stimulated the proliferation of gingival fibroblasts while only concentration 20 µg/ml of exudate induced keratinocyte proliferation (p<0.05).
Conclusion The aloe vera gel extract (1-50 µg/ml) and the exudate extract (20-400 µg/ml) induced the gingival fibroblast proliferation.
(CU Dent J 2002;25:61-70)
Keywords :
Aloe vera; fibroblast; kerationocyte
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