• Pasutha Thunyakitpisal, D.D.S., Ph.D.
• Sirithan Jiemsirilers, BSc., M.S., Ph.D.
• Dujreutai Pongkao Kashima, BSc., M.S., Ph.D.
• Onusa Saravari, BSc., M.S.
• Nonglax Thunyakitpisal, D.D.S., M.S.
• Thitima Rupunt, BSc., M.S.

Objective To evaluate the light-activated pit and fissure resin-based sealant prototypes (LAS-clear and LAS-opaque) for depth of cure, flexural strength, microhardness, and cytotoxicity compared with commercial light-activated pit and fissure resin-based sealants.

Material and methods LAS-clear, LAS-opaque, Delton-clear (DC), Helioseal clear (HC), Helioseal opaque (HO), and Clinpro (CL) were investigated for depth of cure per ISO 6874:2005. Their flexural strength and microhardness were also evaluated. Cytotoxicity was determined using an MTT based colorimetric assay. Statistical analysis was performed using the SPSS program.

Results All materials met ISO 6874:2005 requirements. The mean depth of cure of DC, HC, and LAS-clear were significantly higher than that of HO, CL, and LAS-opaque (p < 0.05). The flexural strength and microhardness values were not significantly different between each group. The MTT assay showed that CL conditioned media significantly reduced cell viability at 24 and 48 hours compared with the control and other sealants (p < 0.05). LAS-clear, LAS-opaque, DC, HC, and HO conditioned media slightly decreased cell viability.

Conclusions LAS-clear and LAS-opaque met the ISO 6874:2005 requirements for depth of cure. LAS-clear and LAS-opaque conditioned medium were biocompatible with gingival fibroblasts.

(CU Dent J. 2016;39:43-52)