The setting up an educational curriculum in dentistry had begun in 1928 by Dr. Wad Yaemprayun (Professor Colonel Luang Vach Vidyavaddhana). He intended to provide Dental education to be taught at the university level in Thailand. After finishing his studies, he received his medical degree from Syracuse University. USA. Support came soon after, in 1925, for studies in the field of dentistry. By that time, a Thai student named Mr. See Sirisingha (Professsor Lieutenant Colonel See Sirisingha) received a scholarship from His Royal Highness, Prince Mahidol of Songkla, to study in the field of dentistry in U.S.A. Both these scholarship recipients played important roles in the founding foe the Faculty of Dentistry in Thailand. 

In 1936, while Professor Colonel Luang Vach Vidyavaddhana was Commander of the Army Medical Corps, Army Region I, approaches were made to Professor Aller Gustin Ellis, President of Chulalongkorn University, about setting up a faculty of dentistry. These initiatives were unsuccessful, Professor Colonel Luang Vach later proposed to the Minister of Defense, Field Marshal P. Pibulsongkram a project to open a school of dentistry which could provide services for military personnel.

At that time permission was granted to the Army Medical Corps to undertake the proposed project. Professor Colonel Luang Vach Vidyavaddhana traveled on assignment to England, France, Germany, the USA and Japan to learn more about what would be needed to set up a school of dentistry. By the time he returned from these study tours, Field Marshal Pibulsongkram had become President of Chulalongkorn University. As a result, the project for the army was shelved in favor of developing a school of dentistry within the university.

In 1940, an independent dentistry section was set up. It began functioning on 16 May, which is regarded as Founder’s Day for what was to become the university’s Faculty of Dentistry. Professor Colonel Luang Vach Vidyavaddhana was appointed first head of the new section.

The School of Dentistry at Chulalongkorn University was the first of such projects in Thailand. The first class of dental students was comprised of 8 persons. At that time, the section did not have its own premises, but found temporary space to conduct classes in the Science Building of the Faculty of Arts and Science. Studies for dental students were taught by Professor Colonel  Luang Vach Vidyavaddhana, Professor Lieutenant Colonel See Sirisingha and Dr. Matin Hanf, a German dentist practicing in Thailand. There were courses in Chemistry, Physiology and Anatomy, which are basic science courses for the medical students.  Dental and medical students took courses together at Siriraj Hospital.

On the 24 of June 1941, the Dental section officially opened its own Dental Building in Soi Chulalongkorn 11 (today this is Soi Chulalongkorn 64) on Phayathai Road. The two-storey building was 11 meters wide and 116.30 meters long and cost 149,000 ฺฺBaht. It was designed by an Italian architect, Professor Ercole Manfredi who was a special tutor at Chulalongkorn’s Faculty of Architecture. When the section had their own space, dental studies were shifted from the Science Building to the new building. In the same year, a physician in private practice, i.e Rong Amato Kunsripisach (Tieb Soomsawasdi), formerly a professor in pathology at Siriraj Hospital, became a special tutor in pathology foe dental students. He continued teaching for 23 years, till his death in 1964

By 1943, the University of Medical Sciences had been instituted. The Faculty of Medicine included Dentistry, Pharmacy and Veterinary sections which had been moved from Chulalongkorn University to the University of Medical Sciences. In the same year, Professor Colonel Luang Vach Vidyavaddhana became the first Dean of the new faculty of Dentistry. In 1944, There were six dental students graduated from this Faculty. The matriculation ceremony took place in the meeting room of the Administration Building of the Faculty of Medicine at Siriraj Hospital.

In 1947 the faculty of Dentistry was officially divided into 12 sections: Administrative, Dental Anatomy, Histology and Dental Pathology; Pharmacology, Operative dentistry, Prosthodontics, Orthodontics, Oral Surgery, Radiology, Anatomy, Physiology and Biochemistry, and the Department of the School of Dental Hygienist (moved from the School of Dental Hygienist of the Ministry of Public Health). In November, 1951 the classrooms. laboratories etc. of the third and fourth year dental students were moved from Building 5 on Rachadamnoen Avenue and installed in the Dental Building at Chulalongkorn Soi 11 where the first and second year dental students were studying.

On the 2 of October, 1956, the faculty opened a hospital to treat dental diseases, receiving funding from the Government Lottery Bureau For use in purchasing a large vehicle to serve as a Mobile dental Unit for the Faculty of Dentistry Hospital.